Lab exercise 6: the smear and simple staining objectives 1 prepare bacterial smears for the microscopic visualization of bacteria 2 perform a simple staining procedure. Lab report for simple stains the lab report on simple stains will be corrected extensively, but not graded it is an opportunity for you to know what is expected in the future, i will not correct . Simple staining is a method of staining in which bacteria are stained by using a single stain simple staining is also called as monochrome staining or positive staining examples of simple stain are methylene blue, safranin, malachite green, basic fuchsin and crystal violet etc. Simple stain simple stains are just that - add one stain to a fixed smear slide, let it sit, rinse it off, let it dry, and view it is a quick procedure for determining the presence and morphology of bacteria in clinical samples such as stool and discharges.
Experiment 2 simple stain and the gram stain in most microbiological staining procedures, the bacteria are first fixed to the slide by the heat fixed smear (figure 1)in this procedure living, potentially pathogenic bacteria are. Staining microscopic specimens a simple stain will generally make all of the organisms in a sample appear to be the same color, even if the sample contains more . Because bacteria are, for the most part, transparent, we use stains to give them color for microscopic observation making a bacterial smear prepares the bacteria to be stained and a simple stain is a quick and easy way to observe bacteria. Since staining of bacterial cells is relatively fast, inexpensive, and simple, it is the most commonly used technique to visualize bacterial cells staining not only makes bacteria more easily seen, but it allows their morphology (eg size and shape) to be visualized more easily.
What is the purpose of simple staining 2 following 9 answers 9 report abuse simple stain and streak plate lab more questions. Negative staining- principle, reagents, procedure and result the main purpose of negative staining is to study the morphological shape, size and arrangement of the bacteria cells that is difficult to stain. Microbiology lab report transcript of simple vs differential stain simple staining vs differential staining by andrea dougherty differential staining simple . The simple stain can be used as a quick and easy way to determine cell shape, size and arrangements of bacteria true to its name, the simple stain is a very simple staining procedure involving single solution of stain.
A simple staining method ziehl-neelsen staining is used to stain species of mycobacterium tuberculosis that do not stain with the standard laboratory staining . Microbiology lab exam 1 stains microbiology lab exam 1 stains by allie_mae, mar 2010 click to rate hated it steps of simple stain 1) stain heat-fixed . Simple staining technique utilizes single basic dye such as crystal violet, methylene blue, basic fuchsin etc all bacteria take up the basic dye uniformly and appear in the same colour only the morphology of the bacteria can be appreciated upon staining.
In a simple staining technique, a positively charged stain colors the negatively charged cells, making them stand out against the light background methylene blue is a simple stain that colors . 3 laboratory report sheet questions: 1 record your results of staining e coli staphylococcus 2 since everything on the slide will be the same color in simple staining, what you. View lab report - lab report 2 – staining technique from fist 1011 at multimedia university, bukit beruang we have used simple staining, .
Provides information regarding size, shape, arrangements, ans staining reactions of microorganisms grown on artifical culture media simple stain (+). A stain with a positive chromogen will stain the bacteria in a simple stain, not the background this is b/c bacterial nucleic acids and certain cell wall components carry a negative charge. The gram stain is a type of differential stain that allows a (micro)biologist to identify the differences between organisms and/or differences within the same organism.